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E2 inhibits uterine sGC mRNA levels in vitro. Uteri were excised from ovariectomized animals and incubated in vitro for 3 h in the presence or absence of E2 (10 nM). Transcript levels for sGC α1 and β1 mRNA were determined by real-time Q-PCR. Mean values ± SEM (n = 4) were normalized to 36b4 and graphed as percent of control.
