Dominant negative effects of 61GA, 61GS, and 62GA on SUR2B + Kir 6.1
current expression. (A) Whole-cell current traces
recorded from cells transiently transfected with SUR2B + Kir 6.1 (WT),
SUR2B + Kir 6.1 + 61GA (61GA), SUR2B + Kir 6.1 + 61GS (61GS), or SUR2B
+ Kir 6.1 + 62GA (62GA) under the control conditions (5 min after
breakin), in the presence of diazoxide (300 μM) and glibenclamide (10
μM), respectively. The pipette solution (see Methods)
was supplemented with 0.5 mM GDP. (B) Summarized data
for the currents measured at −100 mV. P < 0.01
one-way ANOVA.