Skip to main content
. 2001 Jan 2;98(2):729–734. doi: 10.1073/pnas.011370498

Figure 3.

Figure 3

Dominant negative effects of 62GA, 61GA, and 61GS on SUR2B + Kir 6.2 current. (A) Whole-cell current traces recorded from cells transiently transfected with SUR2B + Kir 6.2 (WT), SUR2B + Kir 6.2 + 62GA (62GA), SUR2B + Kir 6.2 + 61GA (61GA), or SUR2B + Kir 6.2 + 61GS (61GS) under the control conditions (5 min after breakin), in the presence of diazoxide (300 μM) and glibenclamide (10 μM), respectively. The pipette solution (see Methods) was supplemented with 3.0 mM ATP. (B) Summarized data for the currents measured at −100 mV. P < 0.01, one-way ANOVA. (C) Mean measured currents at −100 mV recorded from cells transiently transfected with Kir 2.1–His6 and cotransfected with Kir 2.1–His6 and the indicated Kir 6.0 constructs (5 min after breakin).