Figure 6.

14-3-3 proteins bind to DU1 or DU1-like SS. Proteins isolated from digested starch were passed over an anti-14-3-3 column and a control column. Bound proteins were eluted, separated by electrophoresis, transferred to nitrocellulose, and probed with antiserum to ZmSSIII DU1. The anti-GF14 column retained the DU1 cross-reactive protein (largely degraded from multiple processing steps) (lane 1), whereas the negative control column did not (lane 2). Proteins extracted directly from gelled starch were separated by electrophoresis and transferred to nitrocellulose. Probing with biotinylated Zm GF14–12 identified a 14-3-3-binding protein of approximately 200 kDa (lane 3). Probing with antiserum to ZmSSIII DU1 labeled proteins of a similar size (lane 4).