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. 2001 Jan 9;98(2):771–776. doi: 10.1073/pnas.021465298

Figure 2.

Figure 2

(A) Western blot of acd2 mutant plants with anti-ACD2 antiserum. Lanes contain protein from approximately equal weights of leaf tissue. Lanes: 1, Columbia; 2, acd2–2; 3, acd2–5; 4, acd2–6; 5, acd2–8; 6, acd2–12E13; 7, acd2–7. The doublet above the ACD2 band is not detected by antiserum from an independently inoculated rabbit or by affinity-purified antiserum (data not shown); thus, we conclude that it is not specific to the ACD2 protein. (B) ACD2 localizes to chloroplasts and mitochondria. Lanes: 1, crude extract; 2, purified mitochondria; 3, purified chloroplasts. M, mitochondrial form of ACD2; X, chloroplast form of ACD2; CHL, chlorophyll in mg/ml; MITO, mitochondria/microliter.