Abstract
Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled single-stranded DNA generated from a biotinylated single-stranded DNA template, as a long specific primer in the reverse transcription reaction. This new approach allows the detection of transcripts with a low expression level from microgram quantities of total RNA.
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- Green S., Walter P., Kumar V., Krust A., Bornert J. M., Argos P., Chambon P. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature. 1986 Mar 13;320(6058):134–139. doi: 10.1038/320134a0. [DOI] [PubMed] [Google Scholar]
- Nestor P. V., Forde R. C., Webb P., Gannon F. The genomic organisation, sequence and functional analysis of the 5' flanking region of the chicken estrogen receptor gene. J Steroid Biochem Mol Biol. 1994 Aug;50(3-4):121–130. doi: 10.1016/0960-0760(94)90018-3. [DOI] [PubMed] [Google Scholar]