Skip to main content
. 2001 Jan 23;98(3):914–919. doi: 10.1073/pnas.031568098

Figure 4.

Figure 4

Effects of the zwitterionic detergent CHAPS on the hexamerization and ATPase activities of WT Hsp104. (A) Cross-linking assays were performed as in Fig. 1A, in the presence of 2 mM or 10 mM CHAPS. Assembly was inhibited at the higher detergent concentration. (B) Kinetic analysis of WT Hsp104 in the presence of CHAPS. WT Hsp104 (0.02 mg/ml) was incubated for 16 min and Km and Vmax values were calculated as in Fig. 3. In different experiments, the Vmax for ATP hydrolysis was 2- to 4-fold less in 10 mM CHAPS (hexameric, dashed line) than in 2 mM CHAPS (unassembled), whereas Km was changed less than 2-fold.