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Iron EPR signal from whole-cell preparations. Both SO1377 wild-type strain (DSP-10) and mutant (WG3) were cultured in LB liquid for 24 h at 30°C, and EPR samples were prepared as described under "methods". Cell density was adjusted to equal before filling in the quartz EPR tubes according to measured OD600 nm Value of each strain. The major signal appears at g = 4.3.
