Table 1.
Bacterical strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
| E. coli | ||
| S17-1/λpir | RK2 tra regulon, pir, host for pir-dependent plasmids | 26 |
| WG1 | S17-1/λpir strain harboring suicide plasmid pWG3 | This study |
| S. oneidensis | ||
| MR-1 | Wild-type strain | 2 |
| DSP-10 | Spontaneous rif ampin-resistant derivative of the MR-1, used as the reference strain in replace of MR-1 in this study | 11 |
| WG2 | Transconjugant between E. coli WG1 and S. oneidensis DSP-10, containing a chromosomal integration of suicide plasmid pWG3 | This study |
| WG3 | In-frame S01377 gene deletion mutant of 1496-bp, derivative from WG2 | This study |
| Plasmids | ||
| pCVD422 | Suicide plasmid, bla oriT oriR6K sacB | 27 |
| pBSL142 | pBluescript derivative, containing gentamicin -resistance gene | 28 |
| pDC3.O | Suicide plasmid generated by cloning the gentamicin-resistance gene from pBSL142 into the EcoRV site of pCV442 | This study |
| pWG3 | Suicide plasmid generated by cloning a 1.1 kb DNA fragment containing the mutated S01377 gene with 1496-bp deletion into the Sad site of pDS3.0. | This study |