Table 2.
Poly Q sequence patterns and AIB1 amplification level in MCF7 and its variants
| Cell linea | amplification | Sequence patterns | Pattern | (Q)n | Frequency |
| MCF-7 | 22.3 | (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 7/7 |
| MCF-7 P19 | 18.7 | (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 7/9 |
| (CAG)6CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 2 | 29 | 1/9 | ||
| (CAG)5CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 3 | 28 | 1/9 | ||
| MCF-7 P72 | 22.8 | (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 5/7 |
| (CAG)3CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 4 | 25 | 1/7 | ||
| (CAG)3CAA(CAG)7(CAACAG)3(CAACAGCAG)2CAA | 5 | 24 | 1/7 | ||
| LCC1 | 13.5 | (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 7/9 |
| (CAG)3CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 4 | 25 | 1/9 | ||
| (CAG)3CAA(CAG)14(CAACAG)2(CAACAGCAG)2CAA | 6 | 29 | 1/9 | ||
| LCC2 | 14.6 | (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 4/5 |
| CAG(CAACAG)3(CAACAGCAG)2CAA | 7 | 14 | 1/5 | ||
| LCC9 | 15.6 | (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 7/8 |
| (CAG)3CAA(CAG)2CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 8 | 29 | 1/8 | ||
| LY-2 | 19.9 | (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 9/10 |
| (CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 9 | 28 | 1/10 | ||
| R27 | 19.4 | (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 8/9 |
| (CAG)3CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 4 | 25 | 1/9 | ||
| MDA-MB435 | 1.3 | (CAG)3CAA(CAG)2CAA(CAG)11(CAACAG)2(CAACAGCAG)2CAA | 10 | 29 | 2/8 |
| (CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 9 | 28 | 3/8 | ||
| (CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 2/8 | ||
| (CAG)6CAA(CAG)8(CAACAG)2(CAACAGCAG)2CAA | 11 | 26 | 1/8 | ||
| LCC6 | 2.0 | (CAG)3CAA(CAG)2CAA(CAG)10(CAACAG)2(CAACAGCAG)2CAA | 12 | 28 | 1/9 |
| (CAG)3CAA(CAG)2CAA(CAG)6(CAACAG)3(CAACAGCAG)2CAA | 13 | 26 | 1/9 | ||
| (CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 9 | 28 | 3/9 | ||
| (CAG)3CAA(CAG)2CAA(CAG)10(CAACAG)3(CAACAGCAG)2CAA | 14 | 30 | 1/9 | ||
| (CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 15 | 28 | 1/9 | ||
| (CAG)3CAA(CAG)2CAA(CAG)11(CAACAG)2(CAACAGCAG)2CAA | 10 | 29 | 1/9 | ||
| (CAG)3CAA(CAG)2CAACAGCAA(CAG)8(CAACAG)2(CAACAGCAG)2CAA | 16 | 28 | 1/9 | ||
| ZR-75-1 | 1.5 | (CAG)6CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 2 | 29 | 6/7 |
| (CAG)5CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 3 | 28 | 1/7 | ||
| AK-47 | 1.4 | (CAG)6CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 2 | 29 | 2/8 |
| (CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 11 | 28 | 5/8 | ||
| (CAG)5CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 15 | 27 | 1/8 | ||
| A1N4 | 2.0 | (CAG)6CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 2 | 29 | 20/41 |
| (CAG)4CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 17 | 27 | 21/41 |
aLCC1: estrogen independent and responsive which is selected for growth in vivo without estrogens; LCC2: selected from LCC1 by treatment with 4-OH TAM; LCC9: selected from LCC1 by treatment with ICI 182780, resistant to ICI182780 and 4-OH TAM:, LY-2: selected for resistance to 4-OH TAM, KEO and LY 117018; R27: able to grow in the presence of 4-OH TAM. LCC6 is the more aggressive variant of MD-MB435. AK-47 is the variant derived from ZR-75-1. A1N4 is a normal breast cell line. The parental sequence patterns are in bold.