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. 2001 Jan 16;98(3):920–925. doi: 10.1073/pnas.021561498

Figure 3.

Figure 3

Stimulation of RNase P activity by Hsp27. Stimulation by Hsp27 of human nuclear RNase P activity was investigated by mixing 0.115 μg of enzyme purified through a Mono Q column step with different amounts of Hsp27 or 50 ng of BSA. The mixtures were incubated at 37°C (●, Hsp27; ○, BSA) or 43°C (■, Hsp27; □, BSA) for 30 min. After incubation at room temperature for another 30 min, the RNase P activity was assayed at 37°C for 10 min with the use of pSupS1 as substrate (see Materials and Methods).