Figure 5.

A model of the results of the two-hybrid analysis of interactions between protein subunits in human RNase P. The scheme is based on the results of the two-hybrid assay (Table 2) that showed a weak interaction between certain pairs of subunits. No information is available regarding the stoichiometry of subunits, and, therefore, each subunit is represented only once. The subunits shown in red are also in contact with H1 RNA, the RNA subunit of human RNase P (see text). The subunits are designated by their apparent molecular masses (2–4) (N. Jarrous, D. Wesolowski, C. Guerrier-Takada, and S.A., unpublished observations) rather than by their full designations (e.g., p20 rather than Rpp20) to reduce crowding in the figure.