Table 1.

Comparison of MC reduction catalyzed by native MRD and MRD_E55G, and the ligand binding constants of these two proteins

	MRD (native)	MRDE55G
Specific activity (nmol MC/min per nmol protein)	0.22	0.53
Product profile	6	5, 6, 7 (1:16.8:3.3)
Product distribution	Protein bound	Free (∼58%) and protein  bound (∼42%)*
Molar ratio of bound MC to MRD	0.47	0.22
Binding constant for NADH	166.48  ± 50.12 μM	169.56  ± 56.89 μM
Binding constant for MC	40.31  ± 7.49 μM	54.58  ± 4.62 μM

^*Estimated based on the total peak areas of mitosenes in the HPLC analyses.