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. 2001 Jan 23;98(3):932–937. doi: 10.1073/pnas.020472198

Figure 2.

Figure 2

Expression and purification of the recombinant orfD product. (A) SDS/PAGE of the enzyme. Lanes: 1, low molecular mass markers; 2, the pellet of the extract from E. coli M15 (pREP4, pQCLD41) culture after induction with isopropyl β-d-thiogalactopyranoside (IPTG); 3, the supernatant of the same extract; 4, proteins passed through an Ni-nitrilotriacetic acid agarose column; 5, the fraction eluted with 50 mM imidazole; 6, purified enzyme (1 μg) eluted with 200 mM imidazole. (B) Native PAGE of the purified enzyme. Lanes: 1, high molecular mass markers; 2, purified enzyme (0.7 μg).