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. 2001 Jan 23;98(3):932–937. doi: 10.1073/pnas.020472198

Figure 4.

Figure 4

1H NMR spectra of the reaction mixtures. The reaction buffer contained 5 mM MgCl2, 1 mM DTT, 10 μM FMN, 2.5 mM NADPH, and 5 mM IPP. The spectra were obtained after incubation for 12 h at 37°C without (B) and with (A) the orfD product (1 mg). Small signals in ≈3.9–4.8 ppm can be ascribed to NADPH.