Table 1.
Bacterial strains and plasmids used in this study
| E. coli strain and plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DK223 | W3110 dxr∷Kmr | This study |
| E. coli DK331 | W3110 dxr∷Kmr and idi∷Cmr | This study |
| Plasmids | ||
| pTTQ18 | cloning vector; lacIq, Ampr | S. Matsuyama (University of Tokyo) |
| pGEM-11zf(+)aad-1 | contains 2.0-kb Spmr cassette | H. Ikeda (Kitasato University) |
| pTTQ18S | pTTQ18 with 2.0-kb Spmr cassette from pGEM-11zf(+)aad-1 inserted at the ScaI site in the Ampr gene | This study |
| pUMV19 | pUC118 with the 6.8-kb SnaBI-SnaBI fragment (accession no. AB037666) | 25 |
| pUMV20 | pUMV19 with a deletion of the 0.7-kb Sse8387I-Sse8387I fragment | This study |
| pUMV20ΔM | pUMV20 with a deletion of the 0.4-kb MluI-MluI fragment | This study |
| pTMV20S | pTTQ18S with the 6.1-kb XbaI-HindIII fragment from pUMV20 | This study |
| pTMV20SΔM | pTTQ18S with the 5.7-kb XbaI-HindIII fragment from pUMV20ΔM | This study |