Table 2.

FMN and NAD(P)H dependence of the activity of the reconstituted IPP isomerase

Additions to reaction buffer*
FMN or FAD, μM	NAD(P)H, mM	Relative activity, %
FMN	NADPH
0	0	ND
0	0.1	ND
0	0.5	3.6
0	1	6.5
0	5	19
0	10	15
0	20	11
10	0.1	36
10	1	95
10	5	100
10	10	90
10	20	96
	NADH
10	1	92
10	5	97
FAD	NADPH
10	5	51

^*The basal reaction buffer consisted of 0.1 M HEPES (pH 7.0), 2.5 mM MgCl₂, 1 mM DTT, 5 μg enzyme. ND, not detected.