Table 1.
First-order deamidation half times of pentapeptides GlyXxxAsnYyyGly in days at pH 7.4, 37.0°C, 0.15 M Tris⋅HCl
| Yyy
|
|||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gly | His | Ser | Ala | Asp | Thr | Cys | Lys | Met | Glu | Arg | Phe | Tyr | Trp | Leu | Val | Ile | Mean | Median | |
| Xxx | |||||||||||||||||||
| Gly | 1.03 | 9.2 | 11.8 | 21.1 | 28.0 | 39.8 | 40.6 | 48.2 | 50.4 | 73.9 | 57.8 | 64.0 | 63.6 | 77.1 | 104 | 224 | 287 | 70.6 | 50.4 |
| Ser | 0.96 | 8.3 | 15.1 | 24.1 | 30.3 | 45.7 | 60.2 | 55.5 | 54.9 | 59.7 | 59.7 | 52.2 | 64.7 | 76.8 | 110 | 233 | 285 | 72.7 | 55.5 |
| Thr | 1.04 | 9.6 | 17.1 | 24.6 | 27.9 | 50.0 | 55.5 | 57.6 | 47.6 | 60.8 | 51.2 | 76.4 | 80.6 | 72.5 | 110 | 237 | 279 | 74.0 | 55.5 |
| Cys | 1.14 | 10.8 | 19.0 | 26.4 | 30.6 | 48.7 | 46.0 | 46.6 | 64.5 | 48.3 | 83.1 | 73.9 | 83.9 | 111 | 119 | 229 | 304 | 79.1 | 48.7 |
| Met | 1.04 | 10.2 | 15.2 | 22.1 | 26.4 | 43.6 | 49.6 | 60.4 | 56.9 | 72.4 | 58.8 | 61.9 | 74.0 | 92.7 | 113 | 211 | 275 | 73.2 | 58.8 |
| Phe | 1.15 | 10.2 | 18.1 | 24.2 | 27.4 | 39.0 | 46.5 | 58.2 | 58.6 | 62.4 | 61.2 | 69.5 | 75.1 | 102 | 118 | 203 | 287 | 74.2 | 58.6 |
| Tyr | 1.49 | 10.2 | 11.9 | 24.3 | 28.4 | 38.1 | 48.6 | 55.1 | 64.3 | 41.0 | 56.9 | 58.0 | 70.6 | 120 | 118 | 241 | 306 | 76.1 | 55.1 |
| Asp | 1.53 | 9.7 | 17.0 | 24.0 | 29.4 | 52.4 | 54.1 | 75.9 | 57.3 | 46.8 | 87.2 | 70.1 | 70.4 | 80.3 | 111 | 241 | 298 | 78.0 | 57.3 |
| Glu | 1.45 | 9.0 | 16.4 | 25.8 | 32.0 | 36.8 | 44.2 | 77.8 | 59.6 | 60.3 | 80.9 | 70.2 | 94.5 | 98.4 | 130 | 268 | 279 | 81.4 | 60.3 |
| His | 1.14 | 10.7 | 15.7 | 24.6 | 31.2 | 47.2 | 43.9 | 50.2 | 63.1 | 69.4 | 48.9 | 72.1 | 82.3 | 95.4 | 116 | 247 | 327 | 79.2 | 50.2 |
| Lys | 1.02 | 10.5 | 15.6 | 23.6 | 34.0 | 58.1 | 49.0 | 53.5 | 60.9 | 72.5 | 57.4 | 70.1 | 96.7 | 98.1 | 119 | 246 | 313 | 81.1 | 58.1 |
| Arg | 1.00 | 10.0 | 14.3 | 24.4 | 34.7 | 50.7 | 50.5 | 49.6 | 74.4 | 68.3 | 67.4 | 68.3 | 90.0 | 127 | 128 | 247 | 311 | 83.4 | 67.4 |
| Ala | 1.05 | 9.3 | 14.9 | 22.5 | 31.9 | 43.5 | 63.7 | 55.9 | 59.2 | 74.1 | 62.4 | 65.6 | 73.9 | 130 | 124 | 254 | 300 | 81.5 | 62.4 |
| Leu | 1.08 | 10.7 | 16.7 | 25.1 | 32.1 | 46.1 | 53.5 | 60.1 | 62.6 | 56.7 | 62.1 | 72.4 | 75.7 | 74.5 | 155 | 294 | 391 | 87.6 | 60.1 |
| Val | 1.23 | 10.2 | 18.2 | 27.5 | 33.5 | 49.9 | 63.2 | 63.8 | 65.7 | 64.8 | 67.4 | 66.6 | 79.2 | 88.9 | 154 | 291 | 366 | 88.9 | 64.8 |
| Ile | 1.26 | 11.5 | 14.5 | 25.9 | 33.8 | 46.3 | 52.7 | 64.4 | 58.8 | 58.6 | 66.4 | 61.5 | 79.3 | 86.7 | 154 | 295 | 384 | 87.9 | 58.8 |
| Trp | 1.75 | 11.3 | 15.5 | 30.7 | 43.6 | 38.9 | 83.1 | 59.4 | 64.2 | 75.7 | 73.9 | 71.1 | 92.6 | 135 | 133 | 226 | 286 | 84.8 | 71.1 |
| Pro | 1.18 | 12.8 | 18.9 | 31.8 | 48.6 | 63.1 | 60.0 | 67.8 | 78.4 | 92.0 | 72.9 | 100 | 114 | 122 | 181 | 364 | 455 | 111 | 72.9 |
| Mean | 1.20 | 10.2 | 15.9 | 25.1 | 32.4 | 46.5 | 53.6 | 58.9 | 61.2 | 64.3 | 65.3 | 69.1 | 81.2 | 99 | 128 | 253 | 318 | 81.4 | 61.2 |
| SD | 0.05 | 0.25 | 0.50 | 0.65 | 1.4 | 1.7 | 2.4 | 2.1 | 1.8 | 2.9 | 2.6 | 2.4 | 3.1 | 5.0 | 5.0 | 9.3 | 11.9 | 3.1 | 2.4 |
| %SD | 4.5 | 2.4 | 3.2 | 2.6 | 4.2 | 3.6 | 4.5 | 3.6 | 2.9 | 4.6 | 4.0 | 3.4 | 3.8 | 5.0 | 3.9 | 3.7 | 3.7 | 3.7 | 3.7 |
| Median | 1.14 | 10.2 | 15.7 | 24.5 | 31.6 | 46.2 | 51.6 | 57.9 | 60.3 | 63.6 | 62.3 | 69.8 | 79.3 | 97 | 119 | 243 | 302 | 78.5 | 60.3 |