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Fit to five theoretical predictions of the effect of Fb on koff. (A) Arithmetic fit. (B) Logarithmic fit, using the difference between the logs of the predicted and observed koff values. The koff data of tethered neutrophils on P-selectin measured in 0, 3, or 6% Ficoll are plotted vs. the average force on bond, Fb. The pooled data of koff at different viscosities were fit to the indicated theoretical models. DHW, deep harmonic well; SBD, spontaneous bond dissociation.
