Figure 3.

Cyclic redistribution of Gfp-MinE in filamentous cells. Strain PB103/pFX7 [minCDE ⁺/P_lac-minD minE∷gfp] was grown for 4 h in the presence of 10 μM IPTG. Unfixed cells were examined. The culture contained minicells and short- and medium-length filaments, because of overexpression of minDE. A medium-length filament was examined. (Left) Nomarski micrograph. (Right) Fluorescence micrographs taken at 20-s intervals. Polar zones are initially present at both ends of the cell, delimited by MinE rings. Each zone is then displaced toward the cell poles, together with the ring. The polar zones then disappear, and a single long MinE zone appears at midcell. The central zone then collapses toward midcell and the polar zones reappear. R, MinE ring.