Figure 2.

Expression of the hEPOR in the mEPOR locus. mRNA was isolated from bone marrow cells and used as a template for RT-PCR. (A) Relative quantities of mouse and human EPOR transcripts. RT-PCR primers were complementary to both mouse and human EPOR sequences and generated PCR products of the same size; however, only the human EPOR fragment contained an Eco47-III recognition site. For further experimental details, see Materials and Methods. (B) Relative quantities of wthEPOR and mthEPOR mRNAs in wthEPOR/mthEPOR heterozygotes were determined with a Molecular Dynamics PhosphoImager. RT-PCR products were digested with AccI, which specifically recognizes the mthEPOR product. The genotypes of mice and undigested (−) and digested (+) samples are indicated above the blots.