Table 1.
Summary of genes regulated by TGF-β
| GenBank accession no. | Description | Class | Cell lines
|
||
|---|---|---|---|---|---|
| MCF-10A | MCF-10A (Ras/ErbB2) | MDA-MB-231 | |||
| Up-regulated transcripts | |||||
| X58377 | Adipogenesis inhibitory factor* | CK | 3.1 | 3.1 | 16.8 |
| M14083 | β-Migrating plasminogen activator inhibitor-1* | EM | 3.5 | 3.3 | 3.7 |
| J04111 | c-Jun* | TF | 4.4 | 2.4 | 3.3 |
| AB004066 | DEC-1, helix-loop-helix transcription factor | TF | 2.6 | 2.4 | 3.8 |
| J04102 | Erythroblastosis virus oncogene homolog 2 (ets-2) | TF | 3 | 6 | 4.7 |
| X04327 | Erythrocyte 2,3-bisphosphoglycerate mutase† | 4 | 2.6 | 3.1 | |
| X04430 | IFN-β2a | CK | 6.3 | 2.8 | 2.9 |
| U77914 | Jagged | CK | 2.5 | 3.2 | 2.7 |
| M29039 | Jun-B* | TF | 2.6 | 2.7 | 10.4 |
| J03764 | Plasminogen activator inhibitor-1* | EM | 4.5 | 3.5 | 4.5 |
| AF010193 | SMAD7* | ST | 3 | 2.6 | 4.5 |
| AF024710 | Vascular endothelial growth factor (VEGF)* | CK | 2.6 | 2.5 | 2.3 |
| M26576 | Collagen IV* | EM | 2.8 | 2.5 | |
| X06374 | Platelet-derived growth factor α (PDGF-α) | CK | 3.2 | 2.4 | |
| X70683 | Sox-4 | TF | 2.8 | 2.6 | |
| M55152 | Transglutaminase (TGase) | 6.3 | 2.2 | ||
| M85169 | Cytohesin | EM | 2.5 | 2.7 | |
| L07919 | Distal-less homeobox gene (Dlx-2) | TF | 6.5 | 6.8 | |
| AF031167 | IL-15 | CK | 3.1 | 2.4 | |
| X17033 | Integrin α-2 subunit* | EM | 4.3 | 4.6 | |
| X82209 | MN1 | TF | 4.2 | 3.5 | |
| U31201 | Laminin γ2* | EM | 2.9 | 3.6 | |
| V01512 | c-Fos | TF | 2.6 | ||
| X78947 | Connective tissue growth factor* | CK | 4.6 | ||
| L20861 | Wnt-5a | CK | 3.7 | ||
| X02419 | Urokinase plasminogen activator* | EM | 2.6 | ||
| Down-regulated transcripts | |||||
| S73591 | Brain-expressed HHCPA78 homolog | −2.5 | −2.3 | −2.1 | |
| AL049471 | cDNA DKFZp586N012 | −3.4 | −3.1 | −2.9 | |
| U65093 | Msg1-related gene 1 (mrg1) | TF | −3.9 | −2.4 | −7.8 |
| Y00630 | Arg-Serpin (plasminogen activator inhibitor-2) | EM | −2.4 | −3.1 | |
| U45878 | Inhibitor of apoptosis protein-1 | ST | −2.8 | −2.6 | |
| S62539 | Insulin receptor substrate-1 | ST | −2.9 | −3.2 | |
| W29115 | Homo sapiens cDNA | −4 | −2.7 | ||
| AA522530 | Homo sapiens cDNA | −3.9 | −3.8 | ||
| L36463 | Ras interactor (RIN1) | ST | −2.9 | −2.7 | |
| M31166 | Tumor necrosis factor-inducible gene-14 (TSG-14) | CK | −4.9 | −2.5 | |
| V00568 | c-Myc* | TF | −2.8 | ||
| J05008 | Endothelin-1 | CK | −6 | ||
| AB002298 | KIAA0300† | −3 | |||
| X77956 | Id1*† | TF | −3.9 | ||
Genes whose signal was increased or decreased by TGF-β by more than 2-fold in at least three of four experiments are listed (except those labeled †). Fold changes are the average of four experiments. CK, Cytokine growth factor or receptor ligand; TF, transcription factor or modulator; ST, signal transduction molecule; EM, extracellular matrix component or modifier.
*
Previously described TGF-β gene response.
†
Gene response that occurred only after 4 h of TGF-β treatment and was more than 2.5-fold (see text for details).