Skip to main content
. 2001 Jan 30;98(3):1000–1005. doi: 10.1073/pnas.98.3.1000

Figure 3.

Figure 3

Indirect immunofluorescence localization of Aqy2p from Σ1278b and S. chevalieri. (A) aqy1 null cells (JC0023) were fixed, probed with antibodies to Aqy2p, Kar2p (ER resident protein), or Pma1p (plasma membrane protein), and then incubated with fluorescence-conjugated goat anti-rabbit IgG. aqy1-aqy2 double-null cells (JC0177) were labeled with anti-Aqy2p antibodies as a negative control. DAPI staining of the same cells is shown on the right. (B) aqy2 null cells (JC0145) expressing Σ1278b Aqy2p (plasmid pGAQY2), S. chevalieri Aqy2p (pYSchev), or vector without an insert (pYES2) as a negative control. DAPI staining of the cells is shown on the right.