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Three-dimensional stereoscopic reconstitution of PML (red) and SUMO-1 (green) double-stained cell (MCF-7) treated with MG132 showing that although both proteins accumulated in the nucleolus, they do not colocalize with each other any longer. The mathematically deblurred image was generated from a series of 11 optical sections, 0.3 μm apart. DNA staining in blue.
