Skip to main content
. 2001 Jan 30;98(3):1018–1023. doi: 10.1073/pnas.98.3.1018

Figure 4.

Figure 4

Phenotype of rny1Δ strain in the PSY316 background. (A) Cells (PSY316 background) were grown in YPD plates or YPD plus 50 g/liter NaCl plates at 25° or 37°C, as indicated. For complementation experiments, rny1Δ cells were transformed with plasmid containing RNY1, the A. thaliana RNS1 gene, the mammalian gene for pancreatic RNase A, or empty vector (pG1 or YEpWL). (B) WT or rny1Δ cells (PSY316 background) were grown to exponential phase in SD medium at 25°C. Cells were viewed by using differential interference contrast microscopy. v, vacuole. (Bar = 5 μm.) (C) rny1Δ cells were transformed with plasmid containing the mammalian gene for pancreatic RNase A (WT) or the RNase A mutant H119D(H119D) and grown in SD plates at 25° or 37°C, as indicated.