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. 2001 Jan 23;98(3):1036–1041. doi: 10.1073/pnas.021282898

Figure 1.

Figure 1

Release from DNA replication block results in cell cycle progression in the presence of high p53 levels. (A) RKO cells were treated with HU (1.5 mM) for 17 h and then released by extensive washing. At the indicated time points cell extracts were prepared and analyzed by Western blotting using p53 antibody PAb 1801 (p53) or anti-p-S15 (pS15). As a control for pS15 specificity cells were treated with N-acetyl-l-leucinyl-l-leucinyl-N-norleucinal (LLnL) (50 μM) for 2 h. (B) RKO cells were treated as in A and at the indicated time points subjected to cell cycle analysis. NT, not treated; R, release.