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. 2001 Jan 23;98(3):1042–1046. doi: 10.1073/pnas.031562998

Figure 1.

Figure 1

Shear stress increases integrin occupancy on VN and FN. Glass slides were coated with in FN (5 μg/cm2; A) and VN (0.5 μg/cm2; B). HUVECs were plated on these coated slides in the absence of serum for 2 h and then exposed to shear stress (12 dyn/cm2) for 5 and 30 min or kept as static controls. The cells were fixed with 3% paraformaldehyde in PBS for 30 min, permeabilized in 0.2% Triton X-100-PBS for 5 min, and blocked with 10% normal goat serum. (A) The occupied β1 integrin was revealed by immunostaining using HUTS-21 mAb. (B) The occupied β3 integrin was revealed by LIBS-6 mAb. These mAbs were recognized by FITC-conjugated goat anti-mouse IgG (1:100).