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. 2001 Jan 23;98(3):1042–1046. doi: 10.1073/pnas.031562998

Figure 2.

Figure 2

Shear-induced integrin–Shc association is ECM-dependent. Glass slides were coated with FN (5 μg/cm2), CL (6 μg/cm2), LM (5 μg/cm2), or VN (0.5 μg/cm2). HUVECs were seeded on the slides in the absence of serum, and exposed to shear stress (12 dyn/cm2) for 30 min or kept as static controls. (A) The cell lysates were subjected to immunoprecipitation (IP) with LM609 anti-αvβ3, followed by immunoblotting (IB) with anti-Shc pAb. Each pair of lanes represents static control (C) and sheared (S) samples of cells plated on the ECM indicated. αvβ3–Shc association is demonstrated by coimmunoprecipitation in the sheared, but not static, HUVECs on FN and VN. (B) α6β1–Shc association is demonstrated by coimmunoprecipitation in the sheared HUVECs on LM only. Gels shown are representatives of three independent experiments.