Islet hypoplasia in pdx-1(−/−);mTg neonates.
(A) Comparison of pdx-1(−/−);mTg
(smaller) and wild-type (larger) littermates 16 days after birth.
(B) Whole mount of pdx-1(−/−); wtTg
and pdx-1(−/−); mTg mice. Ventral
(Left) and dorsal (Right) views are
included. p, pancreas; d, duodenum; sp, spleen; s, stomach.
(C) Immunocytochemical analysis of insulin (red, arrows)
and glucagon + somatostatin (green, asterisks) cells in sections from
pdx-1(−/−); wtTg (Upper) and
pdx-1(−/−):mTg (Lower).
(D) Whole mount showing smaller developing islets (dark
blue; arrowheads) in pdx-1(−/−) mTg compared with
pdx-1(+/−)
mTgt mice. Blue color indicates expression
of β-galactosidase from the endogenous
pdx-1 allele pdx-1lacZKO
(1).