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. 2001 Jan 16;98(3):1077–1082. doi: 10.1073/pnas.031559298

Figure 1.

Figure 1

The effect of cell passage number and serum starvation on telomerase activity. Telomerase activity was measured in protein extracts (0.5 μg) from adult fibroblast (AF) (passage 4) and FF (passage 1) cell lines (A) and in protein extracts (0.25 μg) from bovine ES-like cells (passage 8) and FF (passage 1) cell lines (C), with the use of the TRAP assay. Heat-inactivated (HI) control, positive 293 cell extract control (+), and negative lysis buffer control (−), are displayed. (B–F) Densitometric analyses of TRAP reaction products displaying the relative telomerase activities between bovine cell samples. Telomerase activity was measured in protein extracts (0.25 μg) from (E) bovine ES cell-like cells and (F) FFs at early passage (EP) (passages 8 and 1, respectively) and late passage (LP) (passages 34 and 24, respectively) and early-passage cells after increasing incubation times under serum starvation (SS) conditions (24 h, 48 h, 5 days, 10 days). Asterisks (*) denote significant differences (P < 0.05) between mean telomerase activities.