Figure 2.

Chemiluminescent detection of telomere length in bovine cells during in vitro culture. (A–C) TRF analysis demonstrating telomere length in early-passage (EP) and late-passage (LP) FFs and bovine ES-like cells cultured under 5% CO₂ in air atmosphere. (A) Telomere length decreased in FF cells from early (4 PD) to late cell passages (33 PD). (B) Telomere length was maintained in the early passages (EP = passage 4; LP = passage 37) of ES-like cell cultures but (C) shortened in ES cells (derived from same starting clone) at very late passages (passage 52; approximately 600–650 population doublings). Molecular weight (MW) markers (lane M) are displayed in kilobases (kb).