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. 2001 Jan 16;98(3):1077–1082. doi: 10.1073/pnas.031559298

Figure 3.

Figure 3

Reprogramming of telomerase activity in cloned bovine fetuses and embryos. Telomerase activity was assessed in protein extracts (0.5 μg) of bovine cell lines and fetuses and in the equivalent of one bovine embryo. (A) Telomerase activity status of bovine adult fibroblasts (AF) and the cloned fetal kidney (cFK) and liver (cFL) tissues produced from the reconstruction of adult fibroblast nuclei with enucleated bovine oocytes. Telomerase activity of fetal kidney (FK) and liver (FL) tissues from a natural mating is shown as positive controls. (B) Comparison of telomerase activity between in vitro-fertilized zygotes (1C) and blastocysts (Blast) with reconstructed embryos (Nuclear Transfer), with FFs as the donor nuclei. (C) Developmental expression of telomerase activity within cloned embryos. Telomerase activity was analyzed in cloned one-cell (1C) and 8- to 16-cell (8–16C) morulae (MR) and blastocysts (BL) reconstructed with nuclei from bovine FFs. (D) Reprogramming of telomerase activity in cloned bovine blastocysts (BL) derived from bovine granulosa cells (GC). Heat-inactivated (HI), positive cell extract control (+), and negative lysis buffer control (−) samples were used in each assay.