Induction of endogenous JA after artificial leaf damage.
Changes in JA levels (mean ± SD) in leaves that were either
wounded artificially by 300 punctures with a needle (diameter 1
mm—this damage corresponded to about 4% missing leaf area and was
spread regularly over the entire leaf surface) once at time 0 (filled
circles) or remained untreated (open circles). Leaves were harvested
after 10, 30, 45, 60, 120, 180, 360, and 600 min (three leaves of
different plants per harvest). (Inset) Dependence of JA
production on damage intensity measured 30 min after artificial damage.
The regression line indicates a saturated dose–response relation
(r2 = 0.64).