Table 1.
Summary of long-term trial 1: parasite mean motility scores and viability (MTT colorimetry)
| Compound/drug and conc. | Mean motility scores over a period of 40 days | Motility | MTT | ||||||||
| DAY | 1 | 5 | 10 | 15 | 20 | 25 | 30 | 35 | 40 | % reduction on day 40 (P) | % inhibition on day 40 (P) |
| Control | 8.0 | 7.4 | 7.1 | 7.0 | 5.3 | 5.5 | 6.3 | 6.4 | 4.9 | ||
| CGP 6140 1.25 × 10-5M (positive control) | 0.5 | 0.8 | 0.0 | 0.5 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 100.0 (0.005) | 83.3 (0.098) |
| Rifampicin 5 × 10-5M | 7.7 | 6.8 | 6.7 | 6.8 | 3.2 | 1.7 | 0.2 | 0.2 | 0.0 | 100.0 (0.002) | 89.6 (0.033) |
| Rifampicin 1.25 × 10-5M | 7.0 | 7.0 | 7.3 | 5.7 | 6.5 | 5.2 | 5.7 | 5.0 | 5.6 | 0.0 (0.647) | 0.0 (0.798) |
| Minocycline 5 × 10-5M | 8.0 | 6.7 | 7.3 | 5.7 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 100.0 (0.002) | 84.1 (0.042) |
| Minocycline 1.25 × 10-5M | 7.8 | 7.5 | 6.7 | 5.7 | 4.3 | 2.5 | 4.8 | 1.7 | 0.0 | 100.0 (0.002) | 93.7 (0.026) |
| Doxycycline 5 × 10-5M | 8.3 | 8.0 | 8.0 | 5.7 | 0.3 | 0.0 | 0.0 | 0.0 | 0.0 | 100.0 (0.002) | 93.0 (0.027) |
| Doxycycline 1.25 × 10-5M | 7.3 | 6.5 | 6.3 | 4.8 | 6.3 | 6.3 | 6.2 | 6.2 | 5.5 | 0.0 (0.699) | 0.0 (0.801) |
| Ethambutol 5 × 10-5M | 6.8 | 6.8 | 6.5 | 6.7 | 4.0 | 4.8 | 4.3 | 5.0 | 2.7 | 44.9 (0.170) | 73.0 (0.108) |
| Dapsone 5 × 10-5M | 7.3 | 7.3 | 7.7 | 5.0 | 6.8 | 7.0 | 7.2 | 7.3 | 6.5 | 0.0 (0.250) | 0.0 (0.798) |
| Pyrazinamide 5 × 10-5M | 7.7 | 7.7 | 7.2 | 3.5 | 5.5 | 5.5 | 5.7 | 5.2 | 3.7 | 24.5 (0.503) | 4.8 (0.949) |
The effects of exposure to a range of antibiotics on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40.