Table 3.
Summary of long-term trial 3: parasite mean motility scores and viability (MTT colorimetry)
| Compound/drug and conc. | Mean motility scores over a period of 40 days | Motility | MTT | ||||||||
| DAY | 1 | 5 | 10 | 15 | 20 | 25 | 30 | 35 | 40 | % reduction on day 40 (P) | % inhibition on day 40 (P) |
| Control | 8.4 | 8.3 | 7.6 | 7.5 | 7.8 | 7.7 | 7.6 | 7.2 | 7.2 | ||
| CGP 6140 1.25 × 10-5M (positive control) | 2.5 | 0.3 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 100.0 (<0.001) | 67.4 (0.0032) |
| Rifampicin 5 × 10-5M | 8.0 | 6.6 | 2.1 | 0.4 | 0.4 | 0.0 | 0.0 | 0.0 | 0.0 | 100.0 (<0.001) | 67.4 (<0.001) |
| Erythromycin 5 × 10-5M | 8.4 | 8.5 | 8.0 | 7.6 | 7.7 | 7.6 | 7.4 | 7.5 | 7.4 | 0.0 (0.539) | 0.0 (0.210) |
| Oxytetracycline 5 × 10-5M | 8.0 | 8.1 | 7.7 | 7.5 | 7.6 | 7.5 | 7.1 | 6.4 | 6.9 | 4.2 (0.360) | 0.0 (0.995) |
| Isoniazid 5 × 10-5M | 8.2 | 8.2 | 7.7 | 8.0 | 7.7 | 7.3 | 6.8 | 5.7 | 5.2 | 27.8 (0.010) | 35.5 (0.074) |
| Isoniazid 5 × 10-5M + Rifampicin 5 × 10-5M | 7.8 | 8.2 | 5.0 | 4.0 | 2.7 | 0.8 | 0.0 | 0.0 | 0.0 | 100.0 (<0.001) | 71.7 (0.002) |
| Trimethoprim 5 × 10-5M | 8.4 | 8.4 | 7.9 | 7.6 | 7.6 | 8.0 | 7.5 | 7.0 | 7.0 | 2.8 (0.549) | 0.0 (0.319) |
| Sulphamethoxazole 5 × 10-5M | 8.1 | 8.3 | 8.4 | 7.6 | 7.6 | 8.0 | 7.5 | 7.3 | 7.1 | 1.4 (0.848) | 0.0 (0.998) |
| Trimeth. 5 × 10-5M + Sulphamethox. 5 × 10-5M | 8.5 | 8.4 | 8.0 | 7.5 | 6.8 | 7.5 | 7.1 | 7.4 | 6.9 | 4.2 (0.449) | 0.0 (0.828) |
| Clofazimine 1.25 × 10-5M | 7.7 | 6.5 | 5.2 | 3.5 | 4.3 | 3.5 | 3.2 | 0.8 | 0.0 | 100.0 (<0.001) | 77.4 (<0.001) |
| Clofazimine 3.13 × 10-6M | 8.2 | 7.0 | 4.5 | 5.5 | 5.0 | 4.8 | 3.4 | 1.2 | 0.8 | 88.9 (<0.001) | 53.3 (0.028) |
The effects of exposure to a range of antibiotics and antibiotic combinations on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40.