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. 2001 Jan 30;98(3):1118–1123. doi: 10.1073/pnas.98.3.1118

Figure 1.

Figure 1

(a) Targeting strategy for the Men1 knockouts. Restriction sites: RV, EcoRV; X, XbaI; R1, EcoRI; n, NdeI; H3, HindIII. Gray triangles, LoxP sites; black bars, probes 8 and 13; arrows, genotyping primers. (b) Southern blot of ES cell genomic DNA digested with EcoRV and probed with probe 8. Wild-type allele (7.9 kb) and targeted allele (3.3 kb) are indicated. (c) Agarose gel electrophoresis of genotype PCRs. Lanes 1–3 are wild-type, heterozygote, and homozygote TSM mice, respectively. Lanes 4–6 are wild-type, heterozygote, and homozygote ΔN3–8 mice, respectively. (d) Lethality in homozygote embryos. (Upper) Wild-type E12.5 embryo on left, TSM homozygote E12.5 embryo on right. (Lower) Wild-type E11.5 embryo on left, ΔN3–8 E11.5 embryo on right.