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. 2006 Mar 31;90(12):4712–4719. doi: 10.1529/biophysj.105.080606

TABLE 1.

Elasticity and shear viscosity of the cytoplasm of different types of cells.

Average viscosity (Poise) Average elasticity at 1 Hz (dyne/cm2) Reference
Single-cell C. elegans embryo 10 ± 1 negligible This work
Serum-starved Swiss3T3 fibroblast* 10 ± 3 50 ± 20 Kole et al. (14)
Serum-starved Swiss 3T3 fibroblast treated with LPA 95 ± 20 120 ± 30 Kole et al. (14)
Serum-starved Swiss3T3 fibroblast subjected to shear flow 300 ± 40 600 ± 50 Lee et al. (13)
Swiss 3T3 fibroblast at the edge of a wound§ 45 ± 15 330 ± 30 Kole et al. (12)
Mouse embryonic fibroblast (MEF) 18 ± 2 140 ± 30 J. S. H. Lee, P. Panorchan, and D. Wirtz, unpublished
HUVEC 17 ± 1 130 ± 10 J. S. H. Lee, P. Panorchan, and D. Wirtz, unpublished

All reported viscosity and elasticity were obtained by particle tracking microrheology. All measurements are mean ± SE. Unit conversions are 1 dyne/cm2 = 0.1 Pa = 0.1 N/m2 = 0.1 pN/μm2. Pa, Pascal; pN, piconewton.

*

Cells placed on fibronectin deposited on glass were serum starved for 48 h before measurements.

Serum-starved cells placed on fibronectin deposited on glass were treated with lysophosphatidic acid (LPA), which was applied 15 min before measurements.

Cells were grown on fibronectin and exposed for 40 min to shear flow before measurements.

§

Cells in complete medium and grown on fibronectin to confluence were wounded to induce migration.

Cells in complete medium and plated on glass.

Cells in complete medium and plated on a peptide hydrogel.