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. 2006 Mar 24;90(12):4662–4671. doi: 10.1529/biophysj.105.079442

FIGURE 8.

FIGURE 8

Minimal evanescent field depth image from a single skeletal muscle fiber labeled with 5′IATR. Image was taken under metal film-enhanced TIRM. Metal film thickness is 30–40 nm. Sarcomeres appear narrower and scattering at the nucleus is minimal compared to the moderate field depth in Figs. 6 or 7. Labeling at the Z-disk is visible but interrupted. Despite the low fluorescence light level, the fiber is cleanly imaged because of the good suppression of background fluorescence by the metal film. The image shows ice crystal formation on the camera window.