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. 2006 Mar 24;90(12):4720–4729. doi: 10.1529/biophysj.105.078725

FIGURE 1.

FIGURE 1

Fast swelling and regulatory volume decrease (RVD) of a Jurkat cell in 100 mOsm trehalose-substituted medium. The photographs show the same cell before (A, isotonic CGM) and after (B–D) an acute hypotonic challenge, at the indicated time intervals. In isotonic CGM (A, t ≤ 0), the cell radius r was 7.4 μm, corresponding to the original isotonic volume V0 ≈ 1.7 pL. Upon medium replacement at time zero, the cell swelled to Vmax ≈ 3.1 pL (r ≈ 9.0 μm) within 2 min (B) and then shrank gradually to its isotonic size within 10–15 min (C–D).