FIGURE 2.

Changes of the relative volume (ν = V/V₀) in Jurkat cells in response to sorbitol- and trehalose-substituted solutions (A and B, respectively) of different osmolalities. The cells were bathed initially (t < 0) in an isotonic saline solution (∼280 mOsm) and then exposed at time zero to solutions having osmolalities of 300, 200, or 100 mOsm as indicated by the numbers close to the data points. Each data point represents the mean ± SE of eight to nine independent determinations. Note that in the presence of trehalose, RVD was observed over the entire hypotonicity range (B, open symbols). RVD also occurred in slightly hypotonic solutions of sorbitol (A, 200 mOsm, open squares), but was completely inhibited at 100 mOsm (A, open circles).