TABLE 1.
Electrical properties of Jurkat cells incubated for 15–30 min in sorbitol- and trehalose-substituted solutions of different osmolalities
| Osmolyte | Sorbitol | Sorbitol | Sorbitol | Trehalose | Trehalose | Trehalose |
|---|---|---|---|---|---|---|
| Osmolality, mOsm | 100 | 200 | 300 | 100 | 200 | 300 |
| Cm, μF/cm2 | 0.73 ± 0.01 | 0.94 ± 0.05 | 1.35 ± 0.04 | 1.42 ± 0.05 | 1.22 ± 0.04 | 1.40 ± 0.05 |
| Gm (mS/cm2) | 9.8 ± 1.5 | n.d. | 3.5 ± 2.9 | 16.2 ± 4.5 | 5.8 ± 3.5 | 8.2 ± 4.1 |
| Number of cells (CFR) | 420 | 120 | 180 | 300 | 300 | 180 |
| σi (mS/cm) | 2.8 ± 0.2 | 5.7 ± 0.3 | 11.0 ± 1.0 | 4.0 ± 0.2 | 5.8 ± 0.5 | 8.4 ± 0.7 |
| ɛi/ɛ0 | 138 ± 5 | 110 ± 9 | 149 ± 12 | 122 ± 18 | 104 ± 12 | 106 ± 10 |
| Number of measurements | 11 | 12 | 12 | 10 | 13 | 9 |
Cm and Gm are the mean values (±SE) determined by curve fitting to the CRF data (Fig. 3). σi and ɛi are the means (±SE) evaluated from the ROT spectra of individual cells (N = 9–13 cells).