FIGURE 6.

Data demonstrating that GalCer domains formed through vesicle fusion display transbilayer asymmetry. (a) Domain height histogram for 250 domains for bilayers formed through quenched and slow-cooling vesicle fusion. The mean domain height under these conditions was 0.9 nm. (b) 1:1 mol ratio of GalCer/DLPC were transferred to each monolayer through Langmuir-Blodgett deposition. This technique resulted in asymmetric, symmetric, and partially symmetric GalCer domains. Domain heights were analyzed for 120 symmetric (dark bars) and asymmetric (white bars) domains. The asymmetric and symmetric height distributions were centered at 0.95 nm and 1.75 nm, respectively.