FIGURE 2.
Reconstitution as monitored by right-angle light scattering at 25°C (open symbols, near the ordinate) and 65°C (solid symbols). Scattering intensity at a wavelength of 632.8 nm, I, versus POPC concentration, cL. Initial SDS concentrations in the cuvette were 0 (squares), 1.0 mM (circles), 1.5 mM (up-triangles), 2.0 mM (down-triangles), 2.5 mM (diamonds), 5.0 mM (left triangles), 7.5 mM (right triangles), and 10 mM (pentagons, breakpoint indicated by arrow). I was registered 3 min after addition of POPC, which was sufficient to attain equilibrium at 65°C but not at 25°C.