FIGURE 8.

Inactivation coincides with a low subconductance current. (A) An excised patch showing a low subconductance state after the initial closure. This is followed by four traces without large-conductance currents (not shown). A pulse to −20 mV opens the channel. (B) A similar recording as in (A) for a cell-attached patch. The potential (p) is here defined as bath potential minus pipette potential. Note that there is a small steady current at the beginning of the pulse at 0 mV. (C) Seven consecutive steps to voltages as indicated. The first trace is from B. (D) The current in the beginning of each trace in C. In traces 2–4 the channel is inactivated.