TABLE 2.
Properties of VDAC and VDAC-like channels from published investigations
| Cell type | VDAC localization | Exp. | [Cl−] (mM) | γ (pS) | γSub (pS) | Volt. dep. | Select. (−/+) | Blockade | Activated by patch excision | |
|---|---|---|---|---|---|---|---|---|---|---|
| Present work | HT22 neuron | pl | PC | 140 | 402 | 28, 220 | bs | >5 | ATP, sucrose, Ab | yes |
| (16) | Rat astrocytes | pl | PC | 145 | 401 | 120, 280 | bs | high | ATP, dextran sulfate | yes |
| (15) | Rat astrocytes | pl | PC | 140 | 434 | bs | high | Ab | yes | |
| (14) | Rat astrocytes | pl | PC | 142 | 300* | 90, 180 | bs | high | L-644,711 | yes |
| (27) | Rat Schwann | pl | PC | 150 | 450 | 220 | bs | 5 | yes | |
| (11) | Rat muscle | pl | PC | 143 | 430 | bs | 5-30 | |||
| (18) | PC12 | pl | PC | 142 | 367 | bs | ||||
| (18) | PC12 | mt | PC | 142 | 321 | bs | ||||
| (6) | Bilayer | mt | BLB | 1000 | 4000 | bs | 2† |
pl, plasma membrane; mt, mitochondrial; Exp., experimental technique; PC, patch clamp; BLB, black lipid bilayer; [Cl−], chloride concentration in the pipette and the bath; γ, single-channel conductance; γSub, subconductance; bs, bell-shaped voltage dependence of open probability; (−/+), anion selectivity divided by cation selectivity; Ab, anti-VDAC antibody.
*
Hypoosmotic solution on one side, which probably reduces the conductance somewhat.
†
5 in physiological solutions.