. Author manuscript; available in PMC: 2006 May 28.

Published in final edited form as: J Biol Chem. 2002 Oct 21;277(52):50828–50833. doi: 10.1074/jbc.M210707200

Table I. EP4 receptor-specific antisense oligonucleotides inhibit CD44 expression.

Cells from each group were incubated with EP4-AS or control 15-mer oligonucleotides (2 μm) for 48 h. CD44 expression was determined by flow cytometry analysis. A significant decrease in CD44 expression was noted following EP4-antisense treatment. The data represent the mean ± S.D. of triplicate determinations in one representative experiment of three separate experiments. Medium alone controls showed CD44 expression that was not significantly different from oligonucleotide control values (data not shown).

	Mean fluorescence intensity
Experimental conditions	Control oligonucleotides	EP4-antisense oligonucleotides
A549-vector	87.5 ± 0.7	50 ± 1.4^a
A549-vector + dmPGE2	181.5 ± 4.9	65.5 ± 7.7^a
A549-sense	138 ± 4.9	49 ± 4.2^a
H157-vector	57.5 ± 11	45 ± 0.0^b
H157-vector + dmPGE2	156 ± 1.4	58.5 ± 0.7^a
H157-sense	488 ± 4.9	255 ± 5.6^a

p < 0.01 compared with control oligonucleotide.

p < 0.05 compared with control oligonucleotide.