Table II. MMP-2-specific antisense oligonucleotides (oligos) inhibit PGE2-mediated CD44 expression.
dmPGE2-treated cells were incubated with MMP-2-AS (AS) or control (Ctrl.) 15-mer oligonucleotides (5 μm) for 30 h. CD44 expression was determined by flow cytometry analysis. PGE2 significantly increased CD44 expression. A significant decrease in CD44 expression was noted following MMP-2-antisense treatment. The data represent the mean ± S.D. of triplicate determinations in one representative experiment of three separate experiments.
| Experimental conditions | Mean fluorescence intensity |
|---|---|
| A549-vector | 166 ± 6.3 |
| A549-vector + dmPGE2 | 235 ± 1.4a |
| A549-vector + dmPGE2 + Ctrl. oligos | 225 ± 22.6 |
| A549-vector + dmPGE2 + AS-oligos | 168 ± 2.1b |
a
p < 0.001 compared with A549-vector control.
b
p < 0.001 compared with control oligonucleotide.