FIG. 1.

Pooled human immunoglobulins contain antibodies reactive with HCMV virion proteins. Cos-7 cells were transfected with plasmids containing HCMV glycoproteins and phosphoproteins, and protein expression was demonstrated by reactivity with mouse monoclonal antibodies (Mouse mab) specific for each protein (Antigen) followed by a Texas Red-conjugated anti-mouse secondary antibody (red signal). Cells were coincubated with pooled human antibodies (Human ab) and detected using a FITC-conjugated anti-human secondary antibody (green signal). The appearance of yellow in each of the merged images (merge) shows colocalization of red and green signals in all rows, indicating reactivity of pooled human antibodies against each of the transfected HCMV proteins. The cell nuclei were stained with Hoechst dye (blue).