FIG. 2.

Affinity-purified human anti-gB and anti-gM/gN antibodies react specifically against gB or gM/gN. HCMV gB, gM/gN, pp65, or pp150 (Antigen) were expressed by transient transfection of Cos-7 cells with plasmids encoding each respective HCMV ORF. Protein expression was confirmed using mouse monoclonal antibodies specific for each protein (Mouse mab), followed by incubation with Texas Red-conjugated anti-mouse secondary antibodies (red signal). Cells were coincubated with affinity-purified human antibodies against either gB (Human anti-gB) (A) or gM/gN (Human anti-gM/gN) (B), and reactivity was detected using a FITC-conjugated anti-human secondary antibody (green signal). Merged images (Merge) show that human anti-gB reacts only with cells transfected with gB antigen (A) and human anti-gM/gN reacts only with cells transfected with gM/gN antigens (B). Hoechst nuclear stain (blue) shows the location of cell nuclei.