FIG. 3.

Affinity-purified human anti-gM/gN antibodies recognize transfected gM alone but not gN. Cos-7 cells were transfected with plasmids expressing either myc-tagged HCMV gM or gN, and protein expression was detected by reactivity with a mouse anti-myc monoclonal antibody followed by a Texas Red-conjugated anti-mouse secondary antibody (Mouse anti-myc mab, red signal). Cells were coincubated with the affinity-purified human anti-gM/gN antibodies used in Fig. 2, and reactivity was detected using a FITC-conjugated anti-human secondary antibody (Human anti-gM/gN, green signal). Merged images (merge) indicate that the human anti-gM/gN antibody recognized gM when expressed alone (yellow), but it had no detectable reactivity against gN (red) when this gene was expressed in the absence of gM.